Figure 2.

Cabazitaxel induced apoptosis and the CCL2‐CCR2 axis inhibited the sensitivity to cabazitaxel. A, DU145, DU145‐TxR and DU145‐TxR/CxR cells (2 × 10⁴ cells/mL) were exposed to 3 nmol/L cabazitaxel for 24 h, and a TUNEL assay was performed. B‐D, DU145, DU145‐TxR and DU145‐TxR/CxR cells (1 × 10⁵) were plated on 6‐well plates. Cells were treated with 3 nmol/L cabazitaxel and different concentrations of human recombinant CCL2 (B) and with 3 nmol/L cabazitaxel and different concentrations of CCR2 antagonist (Ant) (C,D) after being plated for 8 h and were counted at 24, 48 and 72 h (B,C) and at 24 and 48 h (D). Data are shown as means ± SEM (n = 3)