Figure 3.

Incorporation of miR‐144‐5p and miR‐144‐3p into the RNA‐induced silencing complex (RISC) in lung squamous cell carcinoma cell lines. A, Schematic diagram of isolation of RISC‐incorporated microRNAs by Argonaute2 (Ago2) immunoprecipitation. In LUSQ cells, if miR‐144‐5p or miR‐144‐3p regulated gene activities, miR‐144‐5p or miR‐144‐3p was incorporated into the RISC. By carrying out immunoprecipitation with Ago2, which has a central role in the RISC, miR‐144‐5p or miR‐144‐3p incorporated into the RISC was detected. B, Expression levels of miR‐144‐5p following immunoprecipitation by Ago2. C, Expression levels of miR‐144‐3p following immunoprecipitation by Ago2. *P < 0.001