Figure 2.

Western blot analysis of nuclear factor kappa B (NF‐κΒ) p65, IKKα, IκΒα, Cx43, Cx32, Cx26 and RARα expression in colorectal cancer cells. p65 expression was measured in the cytoplasmic, nucleolar, and whole‐cell lysates to determine whether NF‐κΒ translocation to the nucleus is induced by paclitaxel and all‐trans‐retinoic acid (ATRA). “N” are parental cells, “R” are parental cells treated with ATRA for 48 h, “P” are paclitaxel‐resistant cells, “PR” are paclitaxel‐resistant cells treated with ATRA for 48 h, and “C” are Cx43 transfected paclitaxel‐resistant cells. A, Western blot images. β‐Actin is used for cytoplasmic loading control, including p65‐cytoplasm, p65‐whole, IKKα, IκΒα, Cx43, Cx32, and Cx26. Histone H3 is used for nucleolar loading control of p65‐nuclei and RARα. B‐D, Bar diagrams of densitometric analysis where “c” represents cytoplasmic, “n” represents “nucleolar”, and “w” represents whole‐cell lysates. “R”, “P”, “PR” and “C” were compared to “N” using Student's t test. “PR” and “C” were also compared with “P” using Student's t test. *P < .05 represents a significant difference from “N”, and #P < .05 represents a significant difference from “P”. Cx, connexin; RARα, retinoic acid receptor α