Extended Data Fig. 3 |. Electron densities of side chains and putative water molecules of iC++ structure at pH 8.5.

Shown are 2F_o − F_c maps (blue mesh, contoured at 1σ) for ten introduced mutations, and for two putative water molecules as labelled. All ten mutations have well-resolved electron densities to reliably position structural features, which support the interpretation of the roles of introduced mutations.