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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Mol Cancer Res. 2018 Sep 26;17(1):289–298. doi: 10.1158/1541-7786.MCR-18-0364

Figure 2. miR-576–3p does not affect proliferation, but inhibits colony formation.

Figure 2.

(A) The indicated cell lines were transfected with miR-576–3p mimic, miR-576–3p inhibitor, or controls, including a miR-31 mimic and inhibitor as a positive control. MTT assays (quadruplicate) were performed at the indicated intervals; SEM, *P<0.001, two-tailed t-test. (B, C) Colony formation (B) and soft agar (C) assays (both triplicates) were performed with the indicated lung adenocarcinoma cells retrovirally overexpressing miR-576 or scrambled control. Representative images (10×) shown. Data are representative of three independent experiments; SEM; B, A549 *P=7.0×10−3; H460 *P=1.9×10−2; C, *P<0.02, two-tailed t-test.