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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Mol Cancer Res. 2018 Sep 26;17(1):289–298. doi: 10.1158/1541-7786.MCR-18-0364

Figure 4. SGK1 is a direct target of miR-576–3p.

Figure 4.

(A) Schematic of the SGK1 3’-UTR binding site for miR-576–3p. (B) Luciferase activity (triplicates) was measured after transfection of the indicated miRNA mimic or control RNA into 293T cells; SEM; miR-576–3p, *P=8.8×10−4; miR-133b, *P=5.9×10−4, two-tailed t-test. miR-133b and miR-31 mimics served as positive and negative controls, respectively. (C, D) Western blots (C) and qRT-PCR (triplicate, D) were performed with cells transfected with miR-576–3p mimic, miR-576–3p inhibitor, or controls; SEM; Beas2B, *P=0.021; 16HBE, *P=8.7×10−3; H460, *P=8.3×10−3; A549, *P=2.4×10−3, two-tailed t-test. Data are representative of three independent experiments.