(A) The indicated cells were transfected with miR-576–3p mimic, inhibitor, or controls. Phospho-NDRG1 (Thr346) and total NDRG1 levels were determined by Western blot. (B-D) A549 cells were treated with vehicle control (DMSO) or with EMD638683 at the concentrations indicated. Western blots (B) and transwell migration (C) and invasion (D) assays performed (representative images shown, 4×); SEM; C, 10μM *P=0.045, 25μM *P=0.025; D, *P=5.4×10−5, two-tailed t-test. Data are representative of three independent experiments.