Skip to main content
. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Cancer Res. 2018 Nov 6;79(1):61–71. doi: 10.1158/0008-5472.CAN-18-1087

Figure 2. Mutated HRAS, BRAF and ERBB2 can transform Krt6a+ PcSC cells in the hyperplastic lesion of MMTV-Wnt1 mammary glands.

Figure 2.

Tumor-free survival Kaplan-Meier plots of K6a-tva/MMTV-Wnt1 mice with mammary glands intraductally infected by RCAS-HrasQ61L (A), RCAS-BRAFV600E (B), and RCAS-caErbb2 (C), respectively. RCAS-Actb-infected mammary glands were used as negative controls. n = mouse number. The gel picture below A shows that Tumors induced by RCAS-HrasQ61L contain RCAS-HrasQ61L provirus. Genomic DNA extracted from tumors from K6a-tva/MMTV-Wnt1 mice infected by RCAS-HrasQ61L was detected for RCAS-HrasQ61L provirus by PCR. The RCAS-HrasQ61L plasmid was used as positive control (+). The genomic DNA from spontaneous MMTV-Wnt1 tumor was used as negative control (−).