Skip to main content
. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Mol Cancer Ther. 2018 Oct 8;18(1):39–50. doi: 10.1158/1535-7163.MCT-18-0432

Figure 1.

Figure 1.

Identification of Hsp70 and Hsc70 as co-factors bound to GFP-NTDAR in C4–2 cells. A. Representative C4–2-GFP-NTDAR and C4–2-GFP stable sublines were imaged by fluorescence microscopy. B. Western blot analysis of AR, GFP-NTDAR, and GFP expression in 3 C4–2-GFP and 3 C4–2-GFP-NTDAR sublines. AR antibody detects both endogenous AR and transfected GFP-NTDAR. C. Silver staining of proteins precipitated from total cell lysates of 3 C4–2-GFP and 3 C4–2-GFP-NTDAR sublines using anti-GFP beads. D. Skyline analysis for Hsp70 and Hsc70 in data from mass spectrometry. Hsp70 (HSPA1A) and Hsc70 (HSPA8) showed an increase of peptide intensity greater than 50-fold. Each peptide’s intensity (peak area) in each of the 6 samples was shown as the bar.