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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Mol Cancer Ther. 2018 Oct 8;18(1):39–50. doi: 10.1158/1535-7163.MCT-18-0432

Figure 4.

Figure 4.

Effect of Hsp70 knockdown or inhibition on endogenous and transfected AR expression. A. Hsp70 was knocked down by HSPA1A siRNA in C4–2 cells. B. AR-positive C4–2 and AR-negative PC3 cells were transiently transfected with GFP-AR along with GFP as a control. Forty-eight hours after the transfection, the cells were treated with different dose of VER-155008 for 24 hours prior to harvest for Western blot analysis using anti-AR or anti-GFP antibody. C. C4–2 were transfected with Flag-AR or Myc-AR along with GFP expression vector as a control. Twenty-four hours after the transfection, the cells were treated with different dose of VER-155008 for 24 hours prior to harvest for Western blot analysis using anti-Flag, anti-Myc, or anti-GFP antibody.