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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Mol Cancer Res. 2018 Aug 28;17(1):140–152. doi: 10.1158/1541-7786.MCR-18-0199

Figure 2. Inhibition of FASN is associated with a decrease in DH-S1P and S1P in CRC.

Figure 2.

Total level of (A) sphingosine and (B) S1P in NTC and FASNsh HCT116 CRC cells as detected by mass spectrometry (NM-normal medium; SFM-serum free medium; *p < 0.05 vs. control). (C) The effect of TVB-3664 treatment (0.2 μM, 7 days) on the levels of DH-S1P and S1P in cell medium from Pt 93, Pt130 and Pt 2377 PT cells when cultured in NM and SFM conditions. (D) The effect of TVB-3664 treatment (4 weeks, 3 mg/kg) on the levels of DH-S1P and S1P in tumor tissues in Pt 2449 PT PDXs (control n=3; TVB n=3). (C-D) DH-S1P and S1P measurements were performed using HPLC-MS/MS technologies (MUSC Lipidomics Core, SC). Phosphate concentrations were used to normalize samples.