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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Mol Cancer Res. 2018 Aug 28;17(1):140–152. doi: 10.1158/1541-7786.MCR-18-0199

Figure 5. Inhibition of FASN and sphingolipid metabolism decreases migration of primary CRC cells.

Figure 5.

Primary Pt 93 and Pt 2377 PT CRC cells were treated with (A) 0.2 μM TVB-3664 for 7 days and (B) 2.5 μM FTY-720 for 48h and cell migration was assessed using Live Cell Imaging. At least 25 cells from two independent experiments were analyzed. Each line with unique color represents an individual cell’s migration path. Migration potential is analyzed in four parameters: velocity (V), path length (PL), distance from origin (DO) and directionality (D). (C) The effect of FTY-720 treatment (48h) on p-Met, p-Fak and p-Paxilin activation in primary Pt 93, Pt 130, Pt 2377 PT and Pt 2377 LM CRC cells. (D) The effect of overexpression of FASN in Pt 130 cells on cell migration and activation of signaling pathways associated with cell migration. Equal numbers of cells were cultured for 24h prior cell analysis.