Fig. 3.

TAT-SH3 peptide overcomes the inhibition of endogenous Cx43-hemichannels by contractility in primary BCECs. a, b Quantification of the active area of intercellular Ca²⁺ waves in BCECs (a) and ATP release from BCECs (b) provoked by mechanical stimulation in control or thrombin-treated conditions (n = 3). The effect of TAT-SH3 or TAT-SH3 mutant peptides (100 µM) on the active area (a) and the ATP release (b) was determined for control and thrombin-treated conditions. Asterisk indicates a significant difference from the untreated condition, while caret symbol indicates a significant difference from the thrombin-treated control. c Quantification of the ATP release from HeLa cells exogenously expressing Cx43 exposed to [A23187] (0.1, 2 and 10 µM) in the absence or presence of TAT-SH3 (100 µM). Data display ATP release as fold induction of baseline values (n = 5). Asterisk indicates a significant difference between the control and TAT-SH3-treated condition