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. Author manuscript; available in PMC: 2019 Jan 3.
Published in final edited form as: Nature. 2018 Mar 7;555(7696):387–391. doi: 10.1038/nature25748

Figure 2 |. R-loop accumulation in Ewing sarcoma.

Figure 2 |

a, b, Fold-difference in genomic R-loops in IMR90 versus Ewing sarcoma cells (a) and U2OS cells with indicated transfections (b); n = 4 technical replicates. c, Representative immunofluorescence images of nuclei (DAPI), R-loops (S9.6) and nucleoli (nucleolin). Scale bar, 25 μm. d, Thirty-five-kilobase region surrounding the SON gene containing RNA-seq (red), RNAPII ChIP–seq (blue), DRIP–seq (black) and RNaseH-treated (RNH) tracks. Etop, etoposide-treated. Track height represents read counts. e, Immunoblots of indicated replication stress proteins. f, Cell viability response to ATR inhibitor; n = 4 technical replicates. g, Proliferation rate of TC32 cells overexpressing RNaseH1 (RNH1) after ATR inhibition (ATRi). EV, empty vector; n = 3 transfection replicates. Mean ± s.e.m., one-way ANOVA compared to IMR90 control, two-tailed t-test within each cell line. #,*P < 0.05; ##,**P < 0.005, # shows significance compared to control (NT).