Skip to main content
. Author manuscript; available in PMC: 2019 Jan 3.
Published in final edited form as: Nature. 2018 Mar 7;555(7696):387–391. doi: 10.1038/nature25748

Figure 3 |. Functional loss of EWSR1 impairs homologous recombination.

Figure 3 |

a, Representative immunofluorescence images of TP53BP1 and RAD51 foci. Scale bar, 25 μm. b, c, Number of cells with more than five TP53BP1 (b) or RAD51 (c) foci. n = 85 cells, two-tailed t-test. d–h, Homologous recombination (HR) frequency with indicated transfections (efficiency demonstrated in Extended Data Figs 5d, 6d). n = 3 transfection replicates, one-way ANOVA. i, Immunoblots for BRCA1 and EWS–FLI1 in subcellular fractions of TC32 cells with EWS–FLI1 knockdown. Mean ± s.e.m.; #,*P < 0.05; ##,**P < 0.005, # shows significance relative to control (black bar).