Fig. 2.

E2F3d localizes to the OMM. a HeLa cells were transfected with expression vectors encoding Flag-tagged individual E2F3 members and then immunostained with anti-Flag antibody. Scale bar, 20 μm. b HeLa cells were transfected with Flag-tagged WT or deletion mutant E2F3d constructs and incubated with MitoTracker Red CMXRos (200 nM) for 30 min. Cells were then immunostained with anti-Flag antibody. The boxed areas are shown at higher magnification in the lowest panel. Scale bar, 20 μm. c Prediction of TM domains for E2F3d. A hydropathy plot was created using the TMpred algorithm. d Mitochondria were isolated from HeLa cells transfected with a Flag-tagged WT E2F3d construct and treated with proteinase K in the absence or presence of Triton X-100, followed by immunoblotting. e Schematic diagrams of full-length E2F3d (WT) and a series of its deletion mutants. Unique C-terminal domains of E2F3d are highlighted in gray. Full-size scans of immunoblots are shown in Supplementary Information. Please see Supplementary Fig. 1