Table 1.
Effect of p50 phosphomimetic variants on protein dimerization and DNA binding. Percentage peak areas of monomers and dimers for p50 WT, S65D, S242D, S248D and S337D and the mutants. DNA binding dissociation constants KD, and the relative DNA binding affinity with respect to WT p50, are also presented for each p50 variant
| Monomer | Dimer | KD value for DNA binding (nM) | Relative DNA binding affinity | |
|---|---|---|---|---|
| WT | 10.5% | 89.5% | 37 ± 7 | 100% |
| S65D | 9.4% | 90.6% | 140 ± 16 | 26.4% |
| S242D | 12.4% | 87.6% | 424 ± 78 | 8.7% |
| S248D | 5.8% | 94.2% | 112 ± 11 | 33.0% |
| S337D | 25.2% | 74.8% | 111 ± 15 | 33.3% |