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Different buffer tolerances of LILBID-MS and nESI-MS using Avidin and EmrE. Avidin tetramer and EmrE dimer in buffers containing up to 200 mM ammonium acetate were detectable in LILBID-MS (a,c) and nESI (b,d). TRIS (a,b) and HEPES buffer (bottom a–d) are worse to handle for both instruments, requiring increased laser power and CID voltage with increasing buffer concentrations as indicated. All EmrE buffers contain additionally 5× CMC DDM
