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. 2018 Dec 4;29(1):23–41. doi: 10.1038/s41422-018-0113-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Prrc2a deletion in oligodendroglial lineage induces hypomyelination. a, b Body and whole wet brain mass of Prrc2a^f/f;Olig2^Cre+/- and control mice at P28 (two-tailed unpaired Student’s t-test, *P < 0.05, **P < 0.01, f/f, n = 10, f/f; Olig2 n = 8). c T2-weighted MRI examination of 4-week-old mice with indicated genotypes. d Black-gold staining of brain slices from 4-, 8-, and 32-week-old Prrc2a^f/f; Olig2^Cre+/- and control mice. e The quantification of corpus callosum width at the midline of 4-, 8-, and 32-week-old Prrc2a^f/f; Olig2^Cre+/- and control mice (two-tailed unpaired Student’s t-test, *P < 0.05, 4w: n = 6 each group, 8w: n = 5 each group, 32w: n = 5 each group). f TEM analysis of the myelin fibers in the corpus callosum from 4-, 8-, and 32-week-old Prrc2a^f/f; Olig2^Cre+/- and control mice. The white arrowheads indicated the naked axons. g The percentage of myelinated axons in the corpus callosum from 4-, 8-, and 32-week-old mice with indicated genotypes (two-tailed unpaired Student’s t-test, *P < 0.05, ***P < 0.001, 4w: f/f, n = 6, f/f; Olig2-Cre n = 7, 8w: n = 6 each group, 32w: n = 6 each group). h Immunostainings of Pdgfrα (P7), CC1/Olig2, or Sox10 (P28) in corpus callosum from mice with indicated genotypes. The quantification of Pdgfrα⁺, CC1⁺ Olig2⁺, or Sox10⁺ cells was shown in (i) (two-tailed unpaired Student’s t-test, **P < 0.01, ***P < 0.001, Pdgfrα ⁺ cells: n = 7 per group; CC1⁺ Olig2⁺ cells: n = 5 per group, Sox10⁺ cells: n = 5 per group). j GSEA plots evaluating the changes in oligodendroglia linage specific genes in brain tissue samples from 4-week-old Prrc2a^f/f; Olig2^Cre+/- vs. control mice. Note that FDR < 0.25 is statistically significant for GSEA analysis. k Representative GO terms of the biological process categories enriched in transcripts with downregulated expressions from Prrc2a^f/f; Nestin^Cre+/- and Prrc2af^/f; Olig2^Cre+/- mice. l Upper panel shows the heamap of representative DEGs from Prrc2a^f/f; Nestin^Cre+/- and Prrc2af^/f; Olig2^Cre+/, respectively. Bottom panel shows relative gene expression in hippocampus from indicated groups by RT-qPCR analysis (two-tailed unpaired Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001, n = 6 per group)