Fig. 2.

CD1d deficiency attenuates TLR-triggered production of proinflammatory cytokines and type I IFN in macrophages and DCs. a ELISA of cytokines in supernatants of peritoneal macrophages or BMDCs from Cd1d^+/+ and Cd1d^–/– mice either unstimulated (Med) or stimulated with LPS (100 ng/mL), Poly(I:C) (10 μg/mL) or CpG (0.3 μM) for 6 h. b Immunoblot of CD1d expression in macrophages 48 h after transfection with control siRNA (Ctrl) or CD1d siRNA. c ELISA of cytokines in supernatants of macrophages transfected with siRNA as in b, followed by stimulation with LPS, Poly(I:C) or CpG for 6 h. Data are from three independent experiments (a, c; mean ± SEM) or are representative of three independent experiments with similar results (b). *P < 0.05 and **P < 0.01 (Student’s t-test)