Fig. 1.

Acto-myosin contractility inhibition limits centriole separation in a non-synchronous HeLa cells. a Representative pictures of untreated asynchronous HeLa cells expressing Centrin1 (C1-GFP). Blue arrows indicate pair of centrioles. Two different centriole separation behaviors have been observed: in one case (left panel) centrioles separate with distances <2 μm; in the other case (right panel) centrioles separate further than 2 μm, up to 6 μm. Scale bar 10 μm. b Inhibition of acto-myosin contractility via blebbistatin treatment significantly reduces the centriole-to-centriole distance. Red arrows indicate pair of centrioles. Scale bar 10 μm. c Quantification of centriole-to-centriole distance in non-synchronous HeLa cells, untreated (Control) or treated with 10 μM Blebbistatin (Bleb) (Control n = 59 cells; + 10 μM Bleb n = 44 cells). Error bars represent s.e.m.; p-value was obtained with unpaired two-tailed t-test; ****p < 0.0001