Fig. 3.

Acto-myosin force organization differentially controls dynamics of centriole separation. a Selected frames from time-lapse movies of cells expressing C1-GFP (Scale bar = 2 μm). Orange arrows indicate centrioles that maintain a stable distance over time, green indicates an increase in distance, red indicates a decrease in distance. Time is in minutes (min). Images correspond to Supplementary Movies 1–4. b Centriole-to-centriole distance plotted over time for the respective videos. c Centriole-to-centriole distance for all cells and time points (20–30 time points in each cell). d Percentage of time spent open (a centrosome is defined as “open” if centriole-to-centriole distance is above 0.75 μm). e Open time versus centriole-to-centriole distance for all the cells imaged on the micropatterns (Square n = 23 cells; H n = 46 cells; Tripod n = 39 cells; T n = 37 cells). Error bars represent s.e.m.; p-values were obtained with unpaired two-tailed t-test; ****p < 0.0001: **p < 0.001