Ex vivo imaging of DiD-NPs in the main organs and plasma. a Rats were intravenously injected with DiD solution or DiD-NPs of 70, 90, or 110 nm. Blood was collected and organs were harvested 1 h after the administration. b NP accumulation in kidneys. c Imaging of kidneys at different time points after injection of 90-nm DiD-NPs (15 min, 30 min, 1 h, 2 h, and 4 h)