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. 2018 Oct 25;29(1):8–22. doi: 10.1038/s41422-018-0103-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — HBV infection and reactivation in 3D-HepLPCs-Hep. a Extracellular HBV-DNA and secreted viral antigens were monitored from 2 to 10 days post infection in 3D-HepLPCs treated with ETV or TUDC. Error bars represent s.d.; n = 4 donors. b HBsAg staining in 3D-HepLPCs with or without differentiation at 8 days post infection. Scale bars, 25 μm. c QPCR analyses for the expression of cccDNA and 3.5 kb RNA in 3D-HepLPCs with or without differentiation versus HBV-infected PHCs (10 days post infection, n = 4 donors, one-way ANOVA with Dunnett correction for multiple comparisons, n.s., non-significant, **P < 0.01). d Schematic of HBV reactivation in 3D-HepLPCs-Hep derived from HBV-infected donor. e HBsAg staining of the liver tissue collected from HBV-infected donor and f disappearance after 10-day culture in TEM. Scale bars, 50 μm. g Extracellular HBV-DNA and secreted viral antigens are monitored from day 0 to day 30 in HMM + DMSO in 3D-HepLPCs derived from three HBV-infected donors, n = 3 technical replicates. h HBsAg staining of patient-derived 3D-HepLPCs with or without 30-day differentiation. Scale bars, 25 μm. i QPCR analyses for the expression of cccDNA and 3.5 kb RNA in patient-derived 3D-HepLPCs with or without 30-day differentiation versus HBV-infected PHCs (10 days post infection, n = 4 technical replicates, one-way ANOVA with Dunnett correction for multiple comparisons, *P < 0.05, **P < 0.01)