Fig. 6.

The imbalance of Treg and Th17 cells is observed in the AdCD81-induced PE-like rats. a, b Pregnant rats were infected with either AdCD81 or AdCTL on the fifth day of gestation (GD5). The transcriptional levels of FOXP3 and RORC in the placenta from GD6 to GD18 were detected by RT-PCR. c, d The proportions of Treg and Th17 cells were analyzed every 3 days from GD6 to GD18 by FACS (n = 3 at each time point). e The expression of p-P65 and P65 in rat placentas was detected by WB. GAPDH served as an internal control. The relative expression of p-P65/P65 was assessed by ImageJ. f The protein level of IL-6 in rat serum was detected by ELISA. GD gestational day. *P < 0.05, **P < 0.01, ***P < 0.001; ns, no significance