Fig. 4.

TSN induces acute liver injury in mice. C57BL/6 mice were treated with TSN (10 mg/kg) for the indicated time. a Serum ALT and AST activities. b Serum ALP activity. c Serum TBil amount. d Histological evaluation of liver injury induced by TSN. Typical images were chosen from each experimental group (original magnification ×100, upper images; partial enlarged pictures, ×200, a. control; b. TSN 6 h; c. TSN 12 h; d. TSN 24 h; e. 10% PG). e Liver ROS level. f Liver MDA content. g Liver GSH amount. The data are expressed as the mean ± SEM (n = 6–9). *P < 0.05, **P < 0.01, ***P < 0.001 vs. control. PG propylene glycol. Scale bar: 100 μm