Fig. 7.

Quercetin reverses TSN-induced liver injury in mice. C57BL/6 mice were pre-treated with quercetin (Quer; 40, 80 mg/kg) for seven consecutive days and were then administered a single dose of TSN (10 mg/kg) at 1 h after quercetin administration on the seventh day. Mice were killed at 24 h after TSN administration. a Serum ALT/AST activity (n = 8). b Serum ALP activity (n = 7–8). c Liver GSH content (n = 6). d Liver ROS level (n = 6). e Liver MDA content (n = 6). f Hepatic mRNA expression of Nrf2 (n = 3). g Hepatic protein expression of Nrf2. The results represent four repeated experiments. h Quantitative densitometric analysis of Nrf2 (n = 4). i Histological evaluation of liver injury. Typical images were chosen from each experimental group (original magnification, ×100, upper images; partial enlarged pictures, ×200, a. control; b. TSN; c. TSN+Quer 40 mg/kg; d. TSN+Quer 80 mg/kg; e. 10% PC; f. Quer 80 mg/kg). The data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. TSN. PG propylene glycol. Scale bar: 100 μm