Table 2.
Binding affinities of different MLL3 or MLL4 constructs to the histone H4 peptide H411–21
| MLL3/4 constructs | H411–21 peptide Kd (μM) |
|---|---|
| MLL3_(ePHD6)1055–1144 | 35 ± 2 |
| MLL3_(ePHD6)1075–1144 | 31 ± 4 |
| MLL3_(PHD6)1085–1144 | Weak bindinga |
| MLL3_(PHD5-6)1008–1144 | 83 ± 14 |
| MLL3_(PHD5)1009–1055 | NB |
| MLL3_(ePHD3)436–525 | NB |
| MLL4_(ePHD6)1475–1564 | 52 ± 2 |
| MLL4_(ePHD6)1495–1564 | 48 ± 5 |
| MLL4_(PHD6)1505–1564 | NB |
| MLL4_(PHD5)1429–1475 | NB |
Kd values were calculated from single measurement and errors were estimated from fitting curve by Origin software package
NB, no detectable binding
aITC curves cannot be fitted reliably