TABLE 1.
Primers used for NG/17-0503 complete genome sequencing
| Primera | Sequence (5′ to 3′) | Locationb |
|---|---|---|
| HEV-289_f | CTTGGGCCTTGAGTGTGCTA | 4443–4462 |
| HEV-290_f | CCCTATCCAGCGCGTTATACAT | 222–243 |
| HEV-291_r | ACCGACAGTAACCTTGTAGCTG | 1089–1068 |
| HEV-292_r | CATGAGACGGTCCCAGATATGG | 999–978 |
| HEV-293_f | CCTGTGTCGGGTGGAATGAA | 5091–5110 |
| HEV-294_r | GGACTGGTCATACTCGGCAG | 6595–6576 |
| HEV-295_f | ACGAAGGGTCCGATGTTGAC | 1532–1551 |
| HEV-296_r | AATGGCTGGGATCTGGTTCG | 3210–3191 |
| HEV-297_r | GACTCTAGCAGCAGTGTGGG | 3087–3068 |
| HEV-298_f | GATCCCAGCCATTGACTTCGAA | 3198–3219 |
| HEV-299_r | TAGCACACTCAAGGCCCAAG | 4462–4443 |
| HEV-300_r | TGTCGTCAAAAGCATCCCCA | 4351–4332 |
| HEV-302_f | TGCCACTGTAGAACCATGATCC | 1396–1417 |
| HEV-303_r | GGTAGATAAAGCTCATCCCCGG | 2771–2750 |
| HEV-304_r | GCGTCAAAACTAGGACCGATTG | 2729–2708 |
| HEV-311_f | GTGCTATTATGGAGGAGTGCGG | 4457–4478 |
| HEV-312_r | AGCACTATCGAATCATCACCTT | 4694–4673 |
| HEV-321_r | ACAGAGCATAACAAGGCCAGAA | 5983–5962 |
| HEV-322_f | ATGCTGTTGGTGGCTATGCTAT | 5745–5766 |
| HEV-323_r | CCTGGATAACTACACGGGATTCC | 6470–6448 |
| HEV-324_f | CATATCCGGGTCCTATGTGGTAC | 1575–1597 |
| HEV-325_r | GCATCAACYTCCGACCAAGT | 2156–2137 |
| HEV-326_f | GCATGTYTGGGAGTCGGC | 2082–2099 |
a
Forward primer designations end with _f; reverse primer designations end with _r.
b
Numbering is according the HEV prototype strain Burma (GenBank accession no. M73218).