Fig. 5. Quantitative real time polymerase chain reaction results of in the spinal cords of experimental autoimmune encephalomyelitis (EAE)-induced rats with or without Ishige okamurae at peak stage (n=3 per group). I. okamurae suppressed the pro-inflammatory mediator related genes in EAE-induced rats. The mRNA expression levels of tumor necrosis factor-α (TNF-α) (A) and cyclooxygenase-2 (COX-2) (B) in the spinal cords of I. okamurae-treated EAE rats was significantly decreased compared with that of vehicle-treated EAE rats. ^*P<0.05 vs. vehicle treated EAE rats.