Fig. 5. MEHP induces CGC apoptosis in vitro. Cultured cerebellar granule cells (CGCs) were treated with 25, 100 and 250 µM MEHP for 24 h. (A) Representative photomicrographs show the percentage of TUNEL positive CGCs in the four different exposure groups. (B) Corresponding bar graph shows TUNEL positive rate of CGCs in the four groups. (C) Representative photomicrographs show the percentage of CGCs with cleaved caspase-3 positive cells in the four groups. (D) The bar graph shows the percentage of CGCs with cleaved caspase-3 in the four exposure groups. (E) Western blot showing the expression of cleaved caspase-3 after MEHP exposure in vitro (F) Semi-quantitative measurements of cleaved caspase-3 for the four groups. (G) Semi-quantitative measurements of caspase-3 for the four groups. ^*p<0.05 vs control, ^#p<0.05 vs 25 µM MEHP exposure group, & p<0.05 vs 100 µM MEHP exposure group. Scale bar=25 µM. The scale bar is the same for all images in the figure.