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. 2016 Mar 14;2(1):12. doi: 10.3390/gels2010012

Figure 1.

Figure 1

Confocal micrograph showing the effect of cell-generated forces on physisorbed fibronectin. MC3T3 preosteoblasts cultivated for 12 h on a nanograted, O2 plasma-treated PDMS substrate. Fibronectin (10 µm/mL) undergoes extensive remodelling caused by contractile forces. Fibronectin compaction is observed at both ends of actin fibers. Note how fibronectin smears follow the actin direction and leave a dark halo upon compaction. Actin is stained with Tetramethylrhodamine B isothiocyanate-phalloidin (red); fibronectin is stained by immunofluorescence (green). Scale bar: 20 µm.