Figure 7.

Persistent bNAb Secretion In Vivo

Plasma concentrations of secreted PGT128 (A) or VRC01 (B) antibodies detected by ELISA from four different cohorts of humanized mice at the indicated time points. Each symbol represents an individual mouse with solid lines representing the mean for each time point. (C) mRNA expression of PGT128 antibody heavy chain (HC, top), light chain (LC, middle), and actin (bottom) were measured by RT-PCR at 32 weeks in the mock (n = 1, M1), GFP-only (n = 5, G1 to G5), and PGT128 (n = 3, P1 to P3) mice. (D) Same as (C) for VRC01-modified mice, 33 weeks (V2) and 36 weeks: GFP-only (n = 2, G1 and G2), and VRC01 (n = 4, V1 to V4). H2O, water negative control; RT−, no reverse transcriptase; +, bNAb-encoding plasmid positive control.