Figure 2.

CD7-Edited CD7 CAR T Cells Are Cytotoxic against AML Cell Lines

(A) Expression of CD7 in non-treated (NTR) and CD7 and CAR in CD7-edited T cells. (B) Frequency of residual live GFP⁺ KG-1a AML cells upon coculture with control or CD7 CAR T cells for 3 days at a 1:40 effector-to-target (E:T) ratio. (C) Absolute counts of live AML cells at the end of the coculture are plotted on the bar graphs. (D) Absolute counts of residual live KG-1a cells after coculture with CD7 CAR T cells at the indicated E:T ratios. (E) IFNγ production by CD8⁺ and CD4⁺ CD7 CAR T cells upon 4-hr coculture with indicated AML cell lines by intracellular cytokine staining and flow cytometry. Individual data points for each donor are shown. (F) Repeated cytotoxicity against KG-1a AML cells in a sequential killing assay. CD7 CAR T cells were plated with KG-1a AML cells at a 1:4 E:T ratio, and the ratio was restored every 3–4 days after quantification of residual live tumor cells (left) and CAR T cells (right). Data are shown as mean ± SD. Data represent 2–3 independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 by unpaired Student’s t test.