Figure 3.

In Vivo Tracking of Radiolabeled γδ-T Cells

(A) Experiment schedule. (B) Representative SPECT-CT images of MDA-MB-231.hNIS-GFP xenograft NSG mice 24 and 72 hr after ¹¹¹In-labeled PLA administration. (C) Representative PET, SPECT, and CT (merged) scans of a PLA-treated SCID/beige mouse at 1, 48, and 168 hr post-injection of γδ-T cells. Liv, liver; Lu, lungs; Sp, spleen; T, tumor. Endogenous murine NIS expression also results in radiotracer uptake, giving rise to the following signals: La, lacrimal glands; St, stomach; and Thy/Sal, thyroid/salivary glands. (D) Time-activity curves from image-based quantification of ⁸⁹Zr in selected organs. Mean ± SEM of N = 3–4 animals (repeated-measures MM analysis, Bonferroni correction for multiple comparisons). (E) Enlarged maximum intensity projection (MIP), coronal, sagittal, and transversal tumor views (merged PET- and SPECT-CT) in three PLA-treated mice (M1, M2, and M3), 48 hr after γδ-T cell injection.