Fig. 1.

Imaging-based screening. The workflow of an imaging-based RNAi screens typically involves the plating of cells in multi-well plates, and their automated manipulation including addition of siRNA reagents before a specific biological assay is performed. Detection of signal may occur via immunocytochemical methods, by use of genetically encoded markers, or analysis of morphological features. Samples are imaged in a fully automated high-throughput fashion and dedicated image analysis algorithms used to detect and quantitate signals. Secondary data analysis identifies hits based on statistical analysis