Figure 3.

Histopathological Improvements in PMO-Treated Neonatal CXMD_J Skeletal Muscles

(A) Representative H&E-stained sections of various skeletal muscles from PMO-treated CXMD_J dogs, as well as age-matched non-treated CXMD_J and wild-type dogs. Scale bar, 100 μm. (B) Fibrosis and/or necrosis quantification from HE-stained sections of the tibialis anterior (TA) and diaphragm (DIA). n = 3, wild-type and non-treated; n = 6, treated. Error bars, SEM; *p ≤ 0.05, one-tailed Student’s t test. (C) Centrally nucleated fiber count quantification from H&E-stained sections of various skeletal muscles. n = 2–3, wild-type; n = 3, non-treated; n = 6, treated. Error bars, SEM; *p ≤ 0.05, **p ≤ 0.01, one-tailed Student’s t test. For all plots, dots represent individual dogs. Abbreviations: QUA, quadriceps; GAS, gastrocnemius; GRA, gracilis major; IC, intercostal muscles; SC, sternocleidomastoid.