Figure 2.

Changes in Gene Expression after i.m. Administration of Reprogramming pDNA in Nanog-GFP Mice

(A) Nanog-GFP transgenics were i.m. administered, 50 μg pOKS and 50 μg pM in 50 μL 0.9% saline or 50 μL saline alone, into the GA. (B–F) At 2, 4, and 8 days p.i. GA tissues were dissected, and real-time qRT-PCR was performed to determine the relative gene expression of (B) reprogramming factors, (C) endogenous pluripotency markers, (D) genes involved in myogenesis, (E) satellite cell markers, and (F) pericyte markers. Relative expression was normalized to day 2 values (Oct3/4 and GFP mRNAs) or to saline-injected controls (other genes). *p < 0.05 and **p < 0.01 indicate statistically significant differences in gene expression between pDNA and saline-injected groups, assessed by one-way ANOVA or Welch ANOVA. Data are presented as 2ˆ^-ΔΔCt ± propagated error, n = 4.