Table 3.
Primer sequences used for the determination of gene expression by RT-qPCR
| Target Genes | Sequence (5′-3′) | Amplicon Size | Accession Number |
|---|---|---|---|
| Fasn | FWD: TGCTCCCAGCTGCAGGC | 107 | NM_017332 |
| REV: GCCCGGTAGCTCTGGGTGTA | |||
| Lpl | FWD: TTCCTGGATTAGCAGACTCTGTGT | 89 | NM_012598 |
| REV: TCCTGTCACCGTCCATCCAT | |||
| Pparg | FWD: CTCAGTGGAGACCGCCCA | 75 | NM_013124 |
| REV: CAGGGCCTGCAGCAGGT | |||
| Srebf1 | FWD: GATTGCACATTTGAAGACATGCTT | 95 | NM_001276708 |
| REV: CCTGTCTCACCCCCAGCATA | |||
| Lep | FWD: AGACCATTGTCACCAGGATCAAT | 89 | NM_013076 |
| REV: CCCGGGAATGAAGTCCAAA | |||
| Actb | FWD: GACCCAGATCATGTTTGAGACCTT | 79 | NM_031144 |
| REV: AGAGGCATACAGGGACAACACA |
Fasn fatty acid synthase, Lpl lipoprotein lipase, Pparg peroxisome proliferator-activated receptor gamma, Srebf1 sterol regulatory element-binding protein 1, Lep leptin, Actb beta-actin