Fig. 2.

Effect of galectin-3 on MDM efferocytosis in asthma. MDMs from participants with (a) asthma (n = 19) and (b) healthy controls (n = 10), were pre-incubated with 50 μg/mL recombinant galectin-3 for 10 min at 37 °C and co-cultured with PKH-26-labelled apoptotic granulocytes for 90 min at 37 °C. The MDMs were harvested and efferocytosis measured by flow cytometry. MDMs with surface bound but non-internalised granulocytes were excluded using cytochalasin D negative control. The asthma group was split into (c) NEA (n = 7) and (d) EA (n = 8) and efferocytosis examined. Galectin-3 significantly increased efferocytosis in asthma, particularly NEA but not in the healthy controls