Fig. 5.

mTORC1/S6K1-mediated IRS1 inhibition by Tau35. a Western blot analysis of downstream effectors of mTORC1. CHO-FL, CHO-Tau35 and untransfected CHO cell lysates probed with antibodies to phosphorylated/total ribosomal protein S6 kinase beta-1 (S6K1) and phosphorylated/total S6 ribosomal protein (S6). Molecular weight markers (kDa) are shown on the left. b Graphs show the relative amounts of phosphorylated/total S6K1 and phosphorylated/total S6. Data are displayed as percentage change compared to untransfected CHO cells (100%). c Western blot analysis of IRS1 activity. CHO-FL, CHO-Tau35 and untransfected CHO cell lysates probed with antibodies to phosphorylated/total insulin receptor substrate 1 (IRS1), and GAPDH. Molecular weight markers (kDa) are shown on the left. d Graphs show the relative amounts of phosphorylated (Ser636/Ser639)/total IRS1, and phosphorylated (Ser1101)/total IRS1. Data are displayed as percentage changes compared to untransfected CHO cells (100%). Values represent mean ± S.E.M., n = 4–6, one-way ANOVA, *P < 0.05, **P < 0.01