Fig. 3.

HPLC- DAD profiling of Atriplex lasiantha methanol: ethyl acetate extract (AMEA) at different wavelength. Only rutin was detected at 257 nm. Conditions: Mobile Phase A (methanol: acetic acid; deionized water, 100: 20: 180, v/v) and Mobile Phase B (methanol: acetic acid: deionized water, 900: 20: 80,v/v). The flow rate was kept at 1 ml/min. The gradient volume of B was utilized as follows: 0–50% in 0–20 min, 50–100% in 20–25 min and then 100% from 25 to 30 min. The absorption of samples was recorded at different wavelengths e.g. Gallic acid at 257 nm, catechin at 279 nm, Rutin at 257 nm, caffeic acid at 325 nm and kaempferol, quercetin, myricetin at 368 nm, but only rutin was detected