Table 5.
HepG2 Cytotoxicity, Streptomyces hyphae formation inhibition and Anti-leishmanial capability of various solvent extracts
| Extract/ Samples |
Hep G2 Cytotoxicity | Protein kinase inhibition | Antileishmanial capability | |||
|---|---|---|---|---|---|---|
| inhibition% | IC50 (μg/ml) | Diameter of zone of inhibition (mm) | Mortality % | LC50 μg/ml | ||
| Clear zone | Bald zone | |||||
| N | – | – | – | 7 ± 0.37*** | 71 ± 0.49 | 12.8*** |
| C | 10 ± 1.5 | 204*** | – | 8 ± 0.81*** | – | – |
| EA | 18 ± 1.01 | 101*** | – | 9 ± 0.49*** | – | – |
| A | 3 ± 0.9 | 773*** | – | 7 ± 0.97*** | – | – |
| E | 7 ± 0.89 | 312*** | – | 8 ± 0.43*** | – | – |
| M | 21 ± 0.37 | 096*** | – | 9 ± 0.57*** | – | – |
| W | – | – | – | – | 71 ± 0.93 | 16.8*** |
| NEA | – | – | – | 8 ± 0.68*** | 72 ± 0.76 | 11.5** |
| EN | 16 ± 1.34 | 168*** | 8 ± 0.34 | – | – | – |
| MEC | 39 ± 1.01 | 046*** | 9 ± 0.41 | – | – | – |
| MEEA | – | – | 8 ± 0.70 | – | – | – |
| MEA | 12 ± 0.57 | 191*** | – | 11 ± 0.49*** | – | – |
| AW | 15 ± 0.83 | 149*** | 9 ± 0.51 | – | – | – |
| MD | – | – | 8 ± 0.68 | – | – | – |
| PC | 57 ± 2.56 | 5.10 | – | 21 ± 1.02 | 79 ± 1.15 | 0.01 |
Results were presented as mean ± SD; n = 3; The percent potential of each sample has been expressed at 100 μg/mL **: P < 0.01, ***: P < 0.001. -- = Not active, N: n-hexane, C: Chloroform, EA: Ethyl acetate, A: Acetone, E: Ethanol, M: Methanol, W: Distilled water, NEA: n-hexane-Ethyl acetate, EN: ethanol-n-hexane, MEC: methanol-chloroform MEEA: Methanol-Ethyl acetate, MEA: methanol-acetone, AW: acetone-water, MD: methanol-water, PC: Positive control; Doxorubicin employed in cytotoxicity assay, Surfactin in Protein kinase inhibition assay, Amphotericin-B in Antileshmanial assay