Table 1.

Primers used in this study

Target gene	Primer/probe (5'-3')	Type of PCR	Tm (°C)a	Fragment length (bp)
msp4	TCATTCGACATGCGTGAGTCA	Real-time	60	92
	TTTGCTGGCGCACTCACATC		60
	FAM-AGCAGAGAGACCTCGTATGTTAGAGGC-BQ1		70
gltA	GCCGACTTTGTTGCCACTGT	Qualitative	58.7	760
	TCCAACCGCCCTTAGCACAA		59.4
msp4	CCCAGCGTTTCCCTCTGTTA		57.1	497
	GAGTCCGTGGTAGAACCCAC		57.3

^aMelting temperature