Table 1.
Strain numbers | Biochemical identification | Fluorescent PCR | MALDI-TOF MS | 16S rDNA identification | ||||
---|---|---|---|---|---|---|---|---|
Confidence (%) | ID results | Ct values* | ID results | Log score values | ID results | Identity (%) | ID results | |
49 | 99 | GBS | 23 (positive) | GBS | 2.221 | GBS | 100(6)** | GBS |
50 | 98 | GBS | 28 (grey zone) | GBS | 2.478 | GBS | 100(6) | GBS |
51 | 98 | GBS | No Ct | No GBS | 2.317 | GBS | 99(6) | GBS |
53 | 99 | GBS | 27 (grey zone) | GBS | 2.236 | GBS | 100(6) | GBS |
54 | 99 | GBS | 27 (grey zone) | GBS | 2.169 | GBS | 100(6) | GBS |
ATCC 13813 | 99 | GBS | 23 (positive) | GBS | 2.436 | GBS | 100(5) & 99(1) | GBS |
ATCC 19615 | 99 | S.pyogenes | No Ct | No GBS | 2.382 | S.pyogenes | 100(6) | S.pyogenes |
ATCC 25923 | 0 | UNIORG*** | No Ct | No GBS | 2.357 | S.aureus | 100(6) | S.aureus |
*Positive control: < 23, Negative control: =30 or No Ct
**Figures in brackets denote the number of similar sequences
***SMIC/ID-2 cards are dedicated to the identification of streptococcus and cannot identify staphylococci