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. 2019 Jan 3;19:3. doi: 10.1186/s12879-018-3638-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Agarose gel of multiplex allele-specific (MAS)-PCR amplicons obtained from M. tuberculosis H₃₇Rv containing wild-type (I572) sequence (lane Rv) and rifampicin-resistant isolate 5177/06 containing I572F mutation (lane F⁵⁷²) at rpoB codon 572 obtained with NArpoBF, NArpoBR, and NArpoB572F primers. The position of migration of 232 bp and 78 bp fragments are indicated by arrows. Lane M is 100 bp DNA ladder and the positions of migration of 100 bp, 300 bp and 600 bp fragments are marked