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. 2019 Jan 3;10:5. doi: 10.1186/s13287-018-1112-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The content of TNFα in normal tissues and necrotic tissues in patients with steroid-associated ONFH. a The normal tissues (NT) and osteonecrotic tissues (ONT) were obtained. b, c Hematoxylin and eosin staining of the tissues, typical sign of empty lacunae was observed in ONT (magnification, × 200). d Relative levels of TNFα in normal tissues and necrotic tissues. The content of TNFα was measured by ELISA assay and normalized with total proteins. Data is presented as mean ± SD (n = 60, *p < 0.05). e, f Detection of TNFα in bone samples by immunohistochemical staining. Bone samples of NT and ONT were decalcified and sectioned. Anti-TNFα antibody was used for immunohistochemical staining (magnification, × 100)