Fig. 4.

TNFα increases DNA methylation of Runx2 promoter in rMSCs. a Schematic figure indicates 32 CpG sites in CpG island of the rat Runx2 promoter. b DNA methylation status of rat Runx2 promoter in rMSCs treated with or without TNFα using sodium bisulfite sequencing. Each PCR product was subcloned and subjected to nucleotide sequencing analysis. Thirteen representatives of sequenced clones were depicted by filled (methylated) and open (unmethylated) circles for each CpG site. Nine CpG sites could not be detected