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. 2019 Jan 3;10:5. doi: 10.1186/s13287-018-1112-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — ChIP analysis of H3K4me3 and H3K27me3 in rat Runx2 promoter. a Decreased occupancy of H3K4me3 and b increased occupancy of H3K27me3 on rat Runx2 promoter in rMSCs treated with TNFα. The histone modifications of rat Runx2 promoter were analyzed by the ChIP-PCR assay. ChIP was done using anti-H3K4me3 and anti-H3K27me3 monoclonal antibodies, and PCR was performed with specific primers. Normal rabbit IgG was used as loading control. Values are expressed as mean ± SD of three independent experiments. ^##p < 0.01