Fig. 5.

14–3-3ζ/HO-1 complex could regulate STAT3 signaling (a and c) p-STAT3 (Ser-727 and Tyr-705) and total STAT3 levels in the HO-1 knockdown cells (a) and HO-1-overexpressing cells (c) were determined by western blotting. b Immunofluorescent staining of pY-STAT3 in HO-1 knockdown cells. 4′,6-Diamidino-2-phenylindole dihydrochloride staining of the nucleus was done (blue); scale bar denotes 50 μm. d HO-1-overexpressing cells were stably transfected with shNC or sh14–3-3ζ plasmids. pY-STAT3 levels in the indicated cells were analyzed by western blotting. e and g pY-STAT3 levels in 14–3-3ζ knockdown cells (d) and 14–3-3ζ-overexpressing cells (e) were analyzed by western blotting. f Immunofluorescent staining of pY-STAT3 in 14–3-3ζ knockdown cells. h Western blot analysis to qualify pY-STAT3 levels in 14–3-3ζ-overexpressing SK-hep1 cells after being transfected with siHO-1 (or siNC as control) for 48 h